Effects of Different Antibiotics on E.coli Growth

Effects of Different Antibiotics on E.coli Growth The effects of antibiotics on the growth of Escherichia coli have been found in several studies and some recent studies have also focused on the tolerance and reduced growth levels in bacteria to examine the molecular changes that allow such as change. E. Coli and anti-microbial agents: In a study by Dixon et al (2004), the antibacterial effects of microcystin, which is, a cyanotoxin produced by Microcystis aeruginosa have been discussed. In the presence of microcystin, the inhibitory values for a range of hydrophobic inhibitors were significantly reduced. Dixon and his colleagues studied the direct effects of appropriate concentrations of microcystin on the integrity of bacterial inner and outer membranes and found that the presence of microcystin affects the permeability levels of entero-bacterial outer membranes. Tolerance to anti-microbial agents in seen in bacteria, which shows a slower growth rate or which no longer, multiplies. This is very common in the E. coli bacteria, which shows a decreased growth rate after being exposed to antibiotics. In clinical infections bacteria tend to multiply slowly and extended periods of anti-microbial chemotherapy are needed to eradicate these organisms and achieve complete cure. Hu and Coates (2005) used transposon mutagenesis to understand the molecular basis of antibiotic tolerance. The authors screened 5000 Escherichia coli mutants to see reductions of kanamycin tolerance in the late stationery phase and found that 4935 mutants were able to grow to the late stationery phase. The mutant KS639 was most sensitive to kanamycin. This variety of mutant showed an increased sensitivity to kanamycin and gentamicin, ciprofloxacin and rifampicin. From the data obtained it was seen that a mutant lacking intergenic regions showed reduced tolerance to kanamycin. T he studies show that interegenic regions in the E coli may be responsible for anti-microbial agents. In a study that tend to examine the effects of ciprofloxacin on E coli growth, Lueng et al studied the effects of the uptake and release of ciprofloxacin from a hydrophilic stent in an antibiotic solution and the effects of a ciprofloxacin loaded stent in inhibiting the growth of E.coli adherence were tested. The authors immersed segments of (hydrophilic stent) HS in 5 ml of ciprofloxacin solutions for 24 hours and ciprofloxacin remained in solution measure determined the uptake by the HS. CHS (ciprofloxacin-loaded stent) was placed in 5 ml of water for 24 hours and the released ciprofloxacin was measured. CHS was placed on culture plates with E coli and incubated and diameters of the inhibited zones were measured. CHS 0.5 cm in length was incubated in separate 5 ml E coli suspensions. This E coli was measured and compared with control HS. The results showed that zonal inhibition to growth of Escherichia coli was, proportional to the concentration of ciprofloxacin. Accordingly the au thors concluded that there was a free exchange (uptake and release) of ciprofloxacin along a concentration gradient between the antibiotic solution and HS. CHS reduced the number of adhered E coli, but the effect was short-livedà ¢Ã¢â€š ¬Ã¢â€ž ¢. Strains of E. coli and Resistance to Antibiotics: Strains of Escherichia coli that are capable of contaminating raw milk can show heightened resistance to anti-microbial drugs. The susceptibility of E. coli that originates in milk and milk products, meat and several antibiotics such as cotrimoxazole, streptomycin, cephalothin, neomycin and chloramphenicol, erythromycin, ampicillin and amikacin. The minimal inhibition concentrations were detected using a standard micro-dilution method. Babak et al (2004) stated the necessity to identify bacterial strains that have acquired potentially transmissible resistance to anti-microbial drugs. The study by Babak and his colleagues differentiated two kinds of E coli strains, one that is susceptible to the adverse effects of antibiotics and another that is resistant to anti-microbial drugs. There is a global expansion of bacterial resistance to anti-microbial agents such as methicillin and vancomycin with the Staphylococcus aureus showing increased resistance to methicillin and decreased sensitivity to vancomycin. The plague bacillus possesses a plasmid that is transferable to E. coli and has multiple antibiotic resistances. Vancomycin resistant enterococci are constantly transmitted to resistant organisms. These resistant strains have been effectively studied by McCormick (1998) to delineate the antimicrobial-resistant bacterial pathogens. Escherichia coli was found in cattle faeces and novobiocin was used in the isolation method when samples of E coli were separated in different occasions. This study by Tutenel et al (2003) effectively links the isolation of E. coli O157 samples using the antibiotic novobiocin suggesting the adverse effects of antibiotics on bacterial growth or survival. In a recent study by Chartone-Souza et al (2005), a tetracycline-platinum complex was synthesized which was found to be as effective as tetracycline itself in inhibiting bacterial growth of E coli and in this particular study two Escherichia coli sensitive bacterial strains. This tetracycline complex is six times more potent that tetracycline against E Coli HB101/pBR322, a bacterial strain that has developed a resistance to tetracycline. According to Chartone-Souza and others their study is extremely important given the fact that emergent resistance strains of E coli have made it difficult to treat bacterial infections with tetracycline. Conclusion: From the studies discussed above, we see two distinct trends of the effects of antibiotics on the growth of E. coli. Antibiotics can develop increasingly resistant mutant strains of bacteria or can inhibit the growth of a particular strain. Whatever the results are, there have been numerous studies that have substantiated the fact that antibiotics have considerable adverse effects of the growth of E. coli and other bacterial strains.

Gender Disparity a Menace in Society Essay

Gender Disparity a Menace in our society. â€Å"All men were created equal.† How about women then? It is a sad reality that gender is still a basis for inequality in many parts of the world, especially in our nation. India remains the lowest ranked among BRIC countries in the Global Gender Gap index. It ranks 105 out of 135 countries. Health, Education, and Economic participation of women have India at its worst among BRIC countries. It ranks among the lowest when it comes to infant mortality and malnutrition among girls. Almost 8 million and more missing girls with demographers projecting that by the year 2020 there would be 28 to 31 million surplus males in the 15 – 35 age group. The roots of this lie in the very ego men have been holding since the dawn of civilization. Women are equated with sensitive, nurturing, cunning or wily and men with strength, courage, aggressiveness and Dominance amongst others. At least in India, a woman still needs the anchor of a husband. India has witnessed gender inequality from its early history due to its socio-economic and religious practices that resulted in a wide gap between the position of men and women in the society. Clearly, then gender gaps that are widespread in access to basic rights, access to and control of resources, in economic opportunities and also in power and political voice are an impediment to development. There are so many Crimes happening against Women and yet they are to be blamed for the crimes. Rape, sexual harassment even extreme violence is blamed on the woman, for travelling alone, for wearing â€Å"inappropriate† clothes, for daring to cross invisible boundaries. Women have always been considered to carry on the domestic work in our society. From cooking food to caring for the children and old of the house, from washing clothes to cleaning the house†¦. these are the things that our society expects and has been expecting from women. The men are considered the â€Å"Alpha† of the house, they are the only one fit to earn, step out of the doorsteps and experience the â€Å"Masculine† world as we say. But there is another side of the coin, well not so severe as with the women but still, nowadays even men fall prey to this gender disparity. Here are some examples, ridiculing of Male nurses, Stay at home dads, Male domestic servants etc. All of this just makes me think, what have we actually become? Is this what God created us for? To discriminate and subjugate women under such orthodox beliefs, Can women never enjoy the same freedom and security that men have? What we need today are trends where girls are able not only to break out of the culturally determined patterns of employment but also to offer advice about career possibilities that look beyond the traditional pail of Jobs. It is surprising that in spite of so many laws, women still continue to live under stress and strain. To ensure equality of status for our women we still have miles to go, but as it’s said, â€Å"A spark is all that’s needed to ignite a fire, to ignite a revolution of change†. Thus, it is rightly said – Man and Woman are like two wheels of a carriage. The life of one without the other is incomplete.

Christmas Lights On The Square Of A Local Winery Tasting...

Over Thanksgiving break, I sat in a local winery tasting room downtown and watched as the Christmas lights on the square outside turned on for the evening. Melissa Junken, my high school sophomore English teacher, sat across from me sipping her own glass of wine. Her question—the usual-- of â€Å"how’s school going?† resulted in more attitude than I typically give. â€Å"I have to define salvation sometime in the next week... in approximately 6000 words.† It was a blunt statement. I could see the work looming ahead but felt separated from the ideas. I took a sip of my own wine before looking up. Her eyes were wide and she had put her hand on the table. â€Å"What?† I asked. I could feel her waiting for me to say more, or even more so, to show some†¦show more content†¦Hell? Absence of love? Was that hell? Is there hell? Am I saved? How does one gain salvation? Is it gained or â€Å"just there?† All of the questions I struggled to po nder in the earlier weeks rose back to the surface as I struggled to work through these questions on a more personal level. â€Å"What do you think it is?† Her question came sharply through my thoughts and I felt like a deer in the headlights. When I’m in class and speak, my face flames a bright red more times than not. I felt the same heat rise to my face in this moment. â€Å"I don’t know† I tossed out, lamely. We dropped the subject, and she poured us both another glass of wine. I have spent the last month trying to specifically define salvation in entirety. It is complex and complicated in ways that make the question often times more easily avoided or, when answered, over-simplified. In another conversation with Haley Patterson two nights later, she pointed out that we, in fact, do not have the ability to know the answer and as a result, there’s a level of impossibility to defining it. It was a comforting statement—one that made me feel that perhaps I wasn’t as lost as I believed. However, after some time spent mulling it over, I realized I couldn’t agree with it completely. I came back to my working definition of salvation and

Essay about Rules on Formation of Contracts - 1116 Words

In order for a contract to be formed, there are various requirements. These are offer, acceptance, consideration, and the intention to create legal relations. A contract may also be terminated. There are two types of offer; specific and general. Specific offers are those made by one person or group of people who can choose to accept, and general offers are made to a generalised majority, such as in rewards and public advertisements. In the case of CARLILL v CARBOLIC SMOKE BALL, a general offer had been made, as it was a publicised advert. The company did not comply with the terms that it had stated; therefore the court held that the contract had been breached as an offer had been made. It was rightly decided that most offers†¦show more content†¦In BROGDEN v METROPOLITAN RAIL CO it was held the railway company had accepted by placing orders since the amendment of the document, and in TRENTHAM LTD v ARCHITAL LUXFER the court used the reasonable man to identify whether or not there has been acceptance. Both cases seemed to have reasonable outcomes; therefore the courts had been provided with satisfactory rules to help them reach a appropriate verdict. There are various different rules regarding acceptance. There must be a communication of acceptance from the offeree to the offeror. The case of YATES BUILDING v PULLEYN deals whether there had been a prescribed acceptance or not. It was held that there was no practical difference to the offeror therefore the acceptance method was binding. However in the case of ENTORES LTD v MILES FAR EAST CORPORATION there was no prescribed acceptance, yet it was held that the contract was formed in England as that was there acceptance had been received by telex. Other rules that can be used to decide whether there has been acceptance include a waiver of communication of acceptance; silence, which isnt a valid acceptance; ignorance, generally there isnt a binding contract; and acceptance via post using the postal rule. The postal rule can often be misused, as it states that a contract has been formed asShow MoreRelatedRelations Between China And China1367 Words   |  6 PagesConvention on Contracts for the International Sale of Goods (CISG), which establishes a system of uniform sale of goods rules to govern the rights and obligations of parties to international sales contracts by taking into account the differing legal systems and different business expectations common in cross-border transactions. 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